Author: Kolodziejek, Jolanta; Seidel, Bernhard; Jungbauer, Christof; Dimmel, Katharina; Kolodziejek, Michael; Rudolf, Ivo; Hubálek, Zdenek; Allerberger, Franz; Nowotny, Norbert
Title: West Nile Virus Positive Blood Donation and Subsequent Entomological Investigation, Austria, 2014 Document date: 2015_5_11
ID: 0vm2hhdr_16
Snippet: The WNV positive samples were subjected to virus isolation attempts by intracerebral inoculation into suckling mouse brain (SMB), as described earlier [11] . The bacteriologically sterile SMB suspension (designated SMB 1 ) was subsequently used for differential inoculation of adult (5-6 week old) ICR female specific-pathogen-free (SPF) mice. They were injected with 1% infectious SMB 1 suspension intracerebrally (i.c., 0.04 ml, under anesthesia), .....
Document: The WNV positive samples were subjected to virus isolation attempts by intracerebral inoculation into suckling mouse brain (SMB), as described earlier [11] . The bacteriologically sterile SMB suspension (designated SMB 1 ) was subsequently used for differential inoculation of adult (5-6 week old) ICR female specific-pathogen-free (SPF) mice. They were injected with 1% infectious SMB 1 suspension intracerebrally (i.c., 0.04 ml, under anesthesia), 0.2 ml intraperitoneally (i.p.) and 0.2 ml subcutaneously (s.c.), respectively. Each of the three groups consisted of 4 mice.
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