Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing Document date: 2012_11_30
ID: jiy4cp4n_15
Snippet: This system is a solution hybridization-antibody capture technique that uses a chemiluminescence detection system of the hybrid molecules. The DNA from the sample is denatured and then hybridized with a specific RNA probe. The DNA-RNA hybrids are captured by anti-hybrids antibodies. The antibody conjugate is detected with a chemiluminescent substrate and a luminometer serves as a device to measure the light emitted. Hybrid capture assays for dete.....
Document: This system is a solution hybridization-antibody capture technique that uses a chemiluminescence detection system of the hybrid molecules. The DNA from the sample is denatured and then hybridized with a specific RNA probe. The DNA-RNA hybrids are captured by anti-hybrids antibodies. The antibody conjugate is detected with a chemiluminescent substrate and a luminometer serves as a device to measure the light emitted. Hybrid capture assays for detection of human papillomavirus (HPV) and cytomegalovirus (CMV) in clinical specimens are currently commercially available (Digene Corp., Gaithersburg, MD) [12, 13] .
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