Author: Berger, Angela K.; Danthi, Pranav
Title: Reovirus Activates a Caspase-Independent Cell Death Pathway Document date: 2013_5_14
ID: jleccqqx_30
Snippet: Assessment of viral infectivity by indirect immunofluorescence. ATCC L929 cells (5 ϫ 10 4 ) in 96-well plates were adsorbed with 2 PFU/cell of reovirus at room temperature for 1 h. Following incubation at 37°C for 18 h, reovirus-infected cells were visualized by indirect immunofluorescence using an Olympus IX71 fluorescence microscope as described previously (15) . Reovirus antigen-positive cells were quantified by counting fluorescent cells in.....
Document: Assessment of viral infectivity by indirect immunofluorescence. ATCC L929 cells (5 ϫ 10 4 ) in 96-well plates were adsorbed with 2 PFU/cell of reovirus at room temperature for 1 h. Following incubation at 37°C for 18 h, reovirus-infected cells were visualized by indirect immunofluorescence using an Olympus IX71 fluorescence microscope as described previously (15) . Reovirus antigen-positive cells were quantified by counting fluorescent cells in at least two random fields of view in duplicate wells at a magnification of 16ϫ.
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