Title: The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells Document date: 1989_5_1
ID: kjid2e3q_37
Snippet: We previously showed that heterologous cells process proSRIF to mature SRIF and package it into vesicles which enter the regulated pathway (41) . The targeting information could be contained in a structural feature of the proregion, the mature sequence, or the entire prohormone. Computer predictions suggest that proSRIF from several species contains conserved domains (2) which we hypothesize might be important for targeting to the regulated pathw.....
Document: We previously showed that heterologous cells process proSRIF to mature SRIF and package it into vesicles which enter the regulated pathway (41) . The targeting information could be contained in a structural feature of the proregion, the mature sequence, or the entire prohormone. Computer predictions suggest that proSRIF from several species contains conserved domains (2) which we hypothesize might be important for targeting to the regulated pathway. To investigate this possibility, we expressed two chimeric molecules in GH3 cells: PRO-GLO and SIG-GLO. Both chimeras were translocated into the ER as judged by accurate cleavage of the signal peptide. While SIG-GLO rapidly turned over intracellularly and was not secreted, 30% of PRO-GLO traversed the entire secretory pathway. The majority (60%) of this PRO-GLO was cleaved to c~-globin and was targeted to the regulated pathway (45%).
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