Author: Berger, Angela K.; Danthi, Pranav
Title: Reovirus Activates a Caspase-Independent Cell Death Pathway Document date: 2013_5_14
ID: jleccqqx_28
Snippet: Assessment of caspase-3/7 activity. ATCC L929 cells (2 ϫ 10 4 ) were seeded into black, clear-bottom 96-well plates and adsorbed with 10 PFU/ cell of reovirus in serum-free medium at room temperature for 1 h. Following incubation of cells at 37°C for 24 h, caspase-3/7 activity was quantified using the Caspase-Glo-3/7 assay system (Promega)......
Document: Assessment of caspase-3/7 activity. ATCC L929 cells (2 ϫ 10 4 ) were seeded into black, clear-bottom 96-well plates and adsorbed with 10 PFU/ cell of reovirus in serum-free medium at room temperature for 1 h. Following incubation of cells at 37°C for 24 h, caspase-3/7 activity was quantified using the Caspase-Glo-3/7 assay system (Promega).
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