Designing of CD8(+) and CD8(+)-overlapped CD4(+) epitope vaccine by targeting late and early proteins of human papillomavirus

Kaliamurthi, Satyavani; Selvaraj, Gurudeeban; Kaushik, Aman Chandra; Gu, Ke-Ren; Wei, Dong-Qing

The similarity analysis was performed to find out the epitopes that match with the human proteome by using the integrated open database, Protein Information Resource, with peptidematching program. 62, 63 systems biology-based virtual cancer pathway prediction and kinetics A literature survey was performed to collect the required information about the selected protein sequences to design and predict the peptides involved in biochemical pathways. [64] [65] [66] [67] [68] [69] [70] [71] [72] [73] [74] A mathematical and computational model of the entire HPV45 infection signaling pathway interacting with specific genes in the presence of peptides was developed and was visualized using CellDesigner v4.4 modeling tool. 75 Nodes in the pathway represented the entities, and edges represented connectivity of one node to another, which was closely related to each other. 76 The inhibitory concentration (1 µg) of proposed peptides was collected from the published experimental data and pharmacokinetic studies. 64 Mass action kinetics (V=KΠi S) was applied for pharmacokinetic study. Time course and kinetics simulation was performed to concern transition time of cancer and complete Table S1 .