Title: The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells Document date: 1989_5_1
ID: kjid2e3q_7
Snippet: The following plasmids were used: pLJ, a recombinant retrovirus expression vector (15), a gift of Dr. R. Mulligan, The Whitehead Institute, Boston, MA; pSP125E encoding the/~-iactamase signal peptide fused to chimpanzee c~-globin (40), a gift of Dr. V. R. Lingappa, University of California, San Francisco, CA; and pLaSl (13) , containing a 462-bp eDNA encoding anglerfish preproSRIF I to which 5' and Y Eco RI-Bam HI linkers were added (41) . Rabbit.....
Document: The following plasmids were used: pLJ, a recombinant retrovirus expression vector (15), a gift of Dr. R. Mulligan, The Whitehead Institute, Boston, MA; pSP125E encoding the/~-iactamase signal peptide fused to chimpanzee c~-globin (40), a gift of Dr. V. R. Lingappa, University of California, San Francisco, CA; and pLaSl (13) , containing a 462-bp eDNA encoding anglerfish preproSRIF I to which 5' and Y Eco RI-Bam HI linkers were added (41) . Rabbit anti-human hemoglobin antiserum was purchased from Cappel Laboratories (Cochranville, PA).
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