Selected article for: "cdna synthesis and strand cdna synthesis"
Author: Mandapathil, M.; H.Beier, U.; Graefe, H.; Kröger, B.; Hedderich, J.; Maune, S.; Meyer, J. E
Title: Differential chemokine expression patterns in tonsillar disease
  • Document date: 2018_8_23
    • ID: jirwy2kj_5
    Snippet: Frozen tissue samples were ground by mortar, and 1 ml of TRIzol TM reagent (Gibco, Ingolstadt, Germany) was applied upon 200 mg of tissue. Total RNA was isolated following the manufacturer's instructions. After determination of the RNA content using the UVICON-931 UV-spectralphotometer (Kontron, Hamburg, Germany), samples of total RNA were adjusted to 1.0 µg for first strand cDNA synthesis. Quality assessment of the RNA was conducted using a 1% .....
    Document: Frozen tissue samples were ground by mortar, and 1 ml of TRIzol TM reagent (Gibco, Ingolstadt, Germany) was applied upon 200 mg of tissue. Total RNA was isolated following the manufacturer's instructions. After determination of the RNA content using the UVICON-931 UV-spectralphotometer (Kontron, Hamburg, Germany), samples of total RNA were adjusted to 1.0 µg for first strand cDNA synthesis. Quality assessment of the RNA was conducted using a 1% agarose ethidium-bromide stained gel electrophoresis.

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