Selected article for: "dNTP substrate and potential template"
Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage
  • Document date: 2017_9_29
    • ID: k4gtl2o7_40
    Snippet: Next, we analyzed the stimulatory effect of the RNA road-blocking 'model' structure 5 to A6 and to the U6 motifs (Supplementary Figure S6A and B) . RT reactions were also performed using three specific dNTP ratios (1:1, 100:1 and 1:100) for the dNTP substrate specified by the slippage motif and the RNA base adjacent to the motif. LPE analysis showed a similar slippage pattern for A6 as shown for the A7 motif and it also followed the slippage rule.....
    Document: Next, we analyzed the stimulatory effect of the RNA road-blocking 'model' structure 5 to A6 and to the U6 motifs (Supplementary Figure S6A and B) . RT reactions were also performed using three specific dNTP ratios (1:1, 100:1 and 1:100) for the dNTP substrate specified by the slippage motif and the RNA base adjacent to the motif. LPE analysis showed a similar slippage pattern for A6 as shown for the A7 motif and it also followed the slippage rules involving dNTP ratio and potential RNA template structure formation (Supplementary Figure S6 , panels D and E with A6 motif). Interestingly, slippage occurs with the U6 motif and follows the 'slippage rules' (Supplementary Figure S6 , panels D and E with U6 motif).

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