Author: Yang, H-C; Chen, T-L; Wu, Y-H; Cheng, K-P; Lin, Y-H; Cheng, M-L; Ho, H-Y; Lo, S J; Chiu, D T-Y
Title: Glucose 6-phosphate dehydrogenase deficiency enhances germ cell apoptosis and causes defective embryogenesis in Caenorhabditis elegans Document date: 2013_5_2
ID: j3ku7i2c_24
Snippet: Short-term hydrogen peroxide treatment. Staged young adults of mock and G6PD-knockdown C. elegans were harvested and treated with various concentrations of H 2 O 2 prepared in PBS for 30 min at room temperature on a test tube rotator (Snijders, Tilburg, Netherlands). Immediately after the treatment, the worms were pelleted and rinsed with PBS to remove the residual H2O2. The treated C. elegans was transferred to fresh RNAi NGM plate for recovery .....
Document: Short-term hydrogen peroxide treatment. Staged young adults of mock and G6PD-knockdown C. elegans were harvested and treated with various concentrations of H 2 O 2 prepared in PBS for 30 min at room temperature on a test tube rotator (Snijders, Tilburg, Netherlands). Immediately after the treatment, the worms were pelleted and rinsed with PBS to remove the residual H2O2. The treated C. elegans was transferred to fresh RNAi NGM plate for recovery for 2 h followed by picking hermaphrodites for scoring apoptotic germ cells and egg production.
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