Selected article for: "Fc receptor and flow cytometry"
Author: Brabb, Thea; von Dassow, Peter; Ordonez, Nadia; Schnabel, Bryan; Duke, Blythe; Goverman, Joan
Title: In Situ Tolerance within the Central Nervous System as a Mechanism for Preventing Autoimmunity
  • Document date: 2000_9_18
    • ID: kcygxo7h_6
    Snippet: Antibodies. The following monoclonal antibodies were used for flow cytometry: PE-conjugated anti-TCR ␤ (clone H57-597), hamster anti-IgG (clone Ha4/8), rat anti-IgG (clone R35-95), anti-CD3 (clone 17A2), anti-V ␤ 8.1,8.2 (clone MR5-2), and anti-V ␣ 2 (clone B20.1); FITC-conjugated anti-CD44 (clone IM7), anti-IgG (clone R35-95), anti-CD3 (clone 500-A2), anti-CD4 (clone GK1.5), anti-CD8 (clone 53-6.7), anti-TCR ␤ (clone H57.597), and anti-C.....
    Document: Antibodies. The following monoclonal antibodies were used for flow cytometry: PE-conjugated anti-TCR ␤ (clone H57-597), hamster anti-IgG (clone Ha4/8), rat anti-IgG (clone R35-95), anti-CD3 (clone 17A2), anti-V ␤ 8.1,8.2 (clone MR5-2), and anti-V ␣ 2 (clone B20.1); FITC-conjugated anti-CD44 (clone IM7), anti-IgG (clone R35-95), anti-CD3 (clone 500-A2), anti-CD4 (clone GK1.5), anti-CD8 (clone 53-6.7), anti-TCR ␤ (clone H57.597), and anti-CD45RB (clone 16A); biotin-conjugated anti-TCR ␤ (clone H57.597), anti-CD49d (clone R1-2), anti-IgG (clone R35-95), anti-V ␤ 8 (clone MR 5-2), anti-CD45R (clone RA3-6B2), anti-CD8 (clone 53-6.7), anti-V ␣ 2 (clone B20.1), anti-CD4 (clone RM 4-5), anti-CD45RB (clone 16A), and anti-CD62L (clone MEL-14); and purified anti-CD16/CD32 (Fc ␥ III/II receptor, clone 2.4 G2). All monoclonal antibodies were purchased from BD PharMingen except anti-CD3, which was purchased from Caltag. Biotin-conjugated primary monoclonal antibodies were detected by streptavidin-conjugated Tricolor from Caltag.

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