Molecular Characterization of Transcriptome-wide Interactions between Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Porcine Alveolar Macrophages in vivo

Zhou, Ping; Zhai, Shanli; Zhou, Xiang; Lin, Ping; Jiang, Tengfei; Hu, Xueying; Jiang, Yunbo; Wu, Bin; Zhang, Qingde; Xu, Xuewen; Li, Jin-ping; Liu, Bang

In summary, the data presented in this study suggested that during infection with HP-PRRSV Tongcheng piglets exhibited typical clinical signs, but displayed mild regional lung damage at 5 and 7 dpi. Microarray analysis revealed that HP-PRRSV infection has affected PAMs in vivo in expression of the important genes involved in cytoskeleton and exocytosis organization, protein degradation and folding, intracellular calcium and zinc homeostasis. Several potential antiviral strategies might be employed in PAMs, including upregulating IFN-induced genes and increasing intracellular zinc ion concentration. Furthermore, inhibition of the complement system likely attenuated the lung damage during HP-PRRSV infection. This system analysis could lead to a better understanding of the HP-PRRSV-host interaction, and to the identification of novel antiviral therapies and identifying genetic components for swine tolerance/susceptibility to HP-PRRS.