Author: Cho, Sung-Kyu; Kim, Won-Dong
Title: Early diagnosis of radiodermatitis using lactate dehydrogenase isozymes in hairless mice (SKH1-hr) Document date: 2012_12_26
ID: jv1qubm8_11
Snippet: The irradiated SKH1-hr mice were sacrificed by cervical dislocation and 6 tissues − the skeletal muscle, heart, kidney, liver, spleen, and testis − were collected. Each tissue was ground in 3 or 7 volumes (v/w) 0.1 M potassium phosphate buffer (pH 6.85) by using Dounce and Potter-Elvehjem homogenizers in an ice-bath; next, the tissues were centrifuged in a refrigerated centrifuge at 20,000 g for 1 hr (20 PR-52D, Hitachi Koki Co. Ltd., Tokyo, .....
Document: The irradiated SKH1-hr mice were sacrificed by cervical dislocation and 6 tissues − the skeletal muscle, heart, kidney, liver, spleen, and testis − were collected. Each tissue was ground in 3 or 7 volumes (v/w) 0.1 M potassium phosphate buffer (pH 6.85) by using Dounce and Potter-Elvehjem homogenizers in an ice-bath; next, the tissues were centrifuged in a refrigerated centrifuge at 20,000 g for 1 hr (20 PR-52D, Hitachi Koki Co. Ltd., Tokyo, Japan). The supernatants were used as extracts. Blood was collected by cardiac puncture [24] , and serum was obtained after incubation at room temperature for 1 hr and at 4 o C for 6 hrs and centrifugation at 790 g for 20 min.
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