Author: Zhang, Chunsun; Xing, Da
Title: Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends Document date: 2007_6_18
ID: j0bazhy2_26
Snippet: (i) Creation of liquid-liquid two-phase reaction systems. Viovy's group performed continuous-flow, high-throughput PCR amplification using droplets in an immiscible, fluorinated oil/fluorosurfactant solvent, without any detectable contamination between droplets (72, 71) . In such systems, the stability of droplet trains is essential for eliminating cross contamination between droplets. (ii) Use of differential hydrophilic/hydrophobic coating......
Document: (i) Creation of liquid-liquid two-phase reaction systems. Viovy's group performed continuous-flow, high-throughput PCR amplification using droplets in an immiscible, fluorinated oil/fluorosurfactant solvent, without any detectable contamination between droplets (72, 71) . In such systems, the stability of droplet trains is essential for eliminating cross contamination between droplets. (ii) Use of differential hydrophilic/hydrophobic coating.
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