Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing Document date: 2012_11_30
ID: jiy4cp4n_25
Snippet: In the same reaction mixture, two or more primer sets designed for amplification of different targets are used [16] . More than one target sequence in a clinical specimen can be co-amplified in a single tube. However, the primers used must be carefully selected in order that they have similar annealing temperatures and lack complementarity. This kind of PCR is less sensitive than PCR with single primer set. Multiplex PCR assays for viral respirat.....
Document: In the same reaction mixture, two or more primer sets designed for amplification of different targets are used [16] . More than one target sequence in a clinical specimen can be co-amplified in a single tube. However, the primers used must be carefully selected in order that they have similar annealing temperatures and lack complementarity. This kind of PCR is less sensitive than PCR with single primer set. Multiplex PCR assays for viral respiratory pathogens and for detection of viral infections of central nervous system have been developed and commercialized [17, 18] .
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