Author: Holmes, Kathryn V.; Dominguez, Samuel R.
Title: The New Age of Virus Discovery: Genomic Analysis of a Novel Human Betacoronavirus Isolated from a Fatal Case of Pneumonia Document date: 2013_1_8
ID: krvei97r_5
Snippet: What features of the genome and its translational strategy are similar to or different from those of other coronaviruses? Like all coronaviruses, HCoV-EMC has a single-stranded, plus-sense, polyadenylated RNA genome about 30 kb in length. As expected, the 20-kb sequence at the 5= end of the genome is translated to yield a huge polyprotein that is cotranslationally cleaved in cis by two viral proteases into 16 functional nonstructural proteins tha.....
Document: What features of the genome and its translational strategy are similar to or different from those of other coronaviruses? Like all coronaviruses, HCoV-EMC has a single-stranded, plus-sense, polyadenylated RNA genome about 30 kb in length. As expected, the 20-kb sequence at the 5= end of the genome is translated to yield a huge polyprotein that is cotranslationally cleaved in cis by two viral proteases into 16 functional nonstructural proteins that cooperatively form the complex machinery for viral RNA synthesis and RNA recombination. The 10-kb sequence in the 3= region of coronavirus genomes uses a different translational strategy. This region encodes 4 structural proteins with features common to all CoVs as well as several so-called accessory proteins that are different for each coronavirus and whose origins and functions are unknown. A nested series of 3= coterminal polyadenylated subgenomic mRNAs is generated in the cytoplasm, and only the gene at the 5= end of each of these mRNAs is translated. The genome reveals conservation in HCoV-EMC of several potential targets for drugs and vaccines being developed for other CoVs, including the viral spike glycoprotein (S), virus-encoded proteases, and essential enzymatic functions such as the RNA-dependent RNA polymerase and helicase.
Search related documents:
Co phrase search for related documents- accessory protein and end gene: 1
- accessory protein and genome end: 1, 2, 3, 4
- accessory protein and genome end sequence: 1
- accessory protein and helicase RNA dependent RNA polymerase: 1
- accessory protein and nonstructural protein: 1, 2, 3, 4, 5, 6
- accessory protein and potential target: 1, 2
- accessory protein and RNA dependent RNA polymerase: 1, 2, 3
- accessory protein and RNA polymerase: 1, 2, 3
- accessory protein and RNA recombination: 1, 2, 3
- accessory protein and RNA synthesis: 1, 2, 3
- accessory protein and structural protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36
- accessory protein and viral protease: 1, 2
- accessory protein and viral RNA synthesis: 1
- accessory protein and virus encode: 1
- cis cleave and virus encode: 1
- complex machinery and helicase RNA dependent RNA polymerase: 1
- complex machinery and nonstructural protein: 1, 2
- complex machinery and potential target: 1, 2, 3, 4
- complex machinery and RNA dependent RNA polymerase: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date