Designing of CD8(+) and CD8(+)-overlapped CD4(+) epitope vaccine by targeting late and early proteins of human papillomavirus

Kaliamurthi, Satyavani; Selvaraj, Gurudeeban; Kaushik, Aman Chandra; Gu, Ke-Ren; Wei, Dong-Qing

CD8 + and its overlapped CD4 + epitopes of HPV V ( F T F P H A F P F ) D K N G N P 5 7 8 -5 9 3 ( 0 . 7 6 ) a n d Overlapping epitopes Table 3 illustrates the epitope sequence, position in the selected protein, allergenicity, antigenicity, immunogenicity, and IFNγ-producing efficiency of 23 CD8 + T-cell epitopes overlapping CD4 + and B-cell epitopes. All the selected epitopes were found to be immunogenic. Among the 23 epitopes, 19 were identified as non-allergenic and 15 were antigenic with a threshold value of 0.4. In addition, 19 epitopes had the efficiency to induce IFN-γ production. The similarity level zero indicated no unique matches of query epitopes found against the Homo sapiens (Taxonomy ID: 9606). Moreover, these peptides would eliminate the risk of inducing autoimmune disorders.