Selected article for: "cytopathic effect and moi infection"
Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus
  • Document date: 2014_6_16
    • ID: k785hl1r_9
    Snippet: Sf9 cells at a density of 1 × 10 6 per flask were infected with rBV-NA/S1 and rBV-M1 at a multiplicity of infection (MOI) of 5. After 72 h, a marked cytopathic effect was observed in the infected cells. These infected cells were harvested and then analyzed by western blot. Briefly, samples were resolved on 8% gradient polyacrylamide gels, after which fusion protein was detected with chicken polyclonal sera against IBV H120 virus and the M1 prote.....
    Document: Sf9 cells at a density of 1 × 10 6 per flask were infected with rBV-NA/S1 and rBV-M1 at a multiplicity of infection (MOI) of 5. After 72 h, a marked cytopathic effect was observed in the infected cells. These infected cells were harvested and then analyzed by western blot. Briefly, samples were resolved on 8% gradient polyacrylamide gels, after which fusion protein was detected with chicken polyclonal sera against IBV H120 virus and the M1 protein was detected with chicken polyclonal sera against influenza virus H5N1. Horseradish peroxidase (HRP)conjugated donkey anti-chicken secondary antibody (Proteintech Group, USA) was used at a dilution of 1 : 5,000. Sf9 cells were infected with the same amount of wild-type baculovirus and treated in parallel with the recombinant baculovirus infected cells to be used as a negative control.

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