Selected article for: "PCR amplification and Sequencing kit"
Author: Conceição-Neto, Nádia; Theuns, Sebastiaan; Cui, Tingting; Zeller, Mark; Yinda, Claude Kwe; Christiaens, Isaura; Heylen, Elisabeth; Van Ranst, Marc; Carpentier, Sebastien; Nauwynck, Hans J.; Matthijnssens, Jelle
Title: Identification of an enterovirus recombinant with a torovirus-like gene insertion during a diarrhea outbreak in fattening pigs
  • Document date: 2017_9_8
    • ID: kgoczioe_11
    Snippet: First-and second-strand synthesis and random PCR amplification were performed for 17 cycles using a modified Whole Transcriptome Amplification (WTA2) kit procedure (Sigma-Aldrich). Denaturation temperature was increased to 95 C to allow for the denaturation of dsDNA and dsRNA. WTA2 products were purified with MSB Spin PCRapace spin columns (Stratec) and were prepared for Illumina sequencing using the Nextera XT library preparation kit (Illumina)......
    Document: First-and second-strand synthesis and random PCR amplification were performed for 17 cycles using a modified Whole Transcriptome Amplification (WTA2) kit procedure (Sigma-Aldrich). Denaturation temperature was increased to 95 C to allow for the denaturation of dsDNA and dsRNA. WTA2 products were purified with MSB Spin PCRapace spin columns (Stratec) and were prepared for Illumina sequencing using the Nextera XT library preparation kit (Illumina). Libraries were quantified with the KAPA Library Quantification kit (Kapa Biosystems) and sequencing of the samples was performed on a HiSeq TM 2500 platform (Illumina) for 300 cycles (150 bp paired ends), generating 67,251,870 reads.

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