Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus Document date: 2014_6_16
ID: k785hl1r_23
Snippet: Serum samples were heat-inactivated at 56 o C for 30 min, then two-fold serially diluted. The diluted serum samples were mixed with IBV H120 viruses (100xTCID 50 ; TCID, tissue culture infective dose) and incubated at 37 o C under 5% CO 2 for 1h. The mixture of serum and H120 virus was inoculated into 9-day-old SPF embryonated chicken eggs. The neutralization titers were expressed as the reciprocal of the highest serum dilution that neutralized 1.....
Document: Serum samples were heat-inactivated at 56 o C for 30 min, then two-fold serially diluted. The diluted serum samples were mixed with IBV H120 viruses (100xTCID 50 ; TCID, tissue culture infective dose) and incubated at 37 o C under 5% CO 2 for 1h. The mixture of serum and H120 virus was inoculated into 9-day-old SPF embryonated chicken eggs. The neutralization titers were expressed as the reciprocal of the highest serum dilution that neutralized 100xTCID 50 IBV H120 in 50% of the SPF embryonated chicken eggs.
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