Author: Mordecai, Gideon J; Wilfert, Lena; Martin, Stephen J; Jones, Ian M; Schroeder, Declan C
Title: Diversity in a honey bee pathogen: first report of a third master variant of the Deformed Wing Virus quasispecies Document date: 2015_11_17
ID: k2n6ropo_9
Snippet: Sequencing and assembly Total RNA was sequenced without an amplification step. Illumina (San Diego, CA, USA) Hi-seq (2 × 100) pair-end sequencing was carried out by TGAC (The Genome Analysis Centre) and at the University of Exeter on four samples from Highfield et al. (2009) ; Late June, Early October and Late October from GD1, and early October from GD2 (Supplementary Table S1 ). The samples were originally collected from Devon in the southwes.....
Document: Sequencing and assembly Total RNA was sequenced without an amplification step. Illumina (San Diego, CA, USA) Hi-seq (2 × 100) pair-end sequencing was carried out by TGAC (The Genome Analysis Centre) and at the University of Exeter on four samples from Highfield et al. (2009) ; Late June, Early October and Late October from GD1, and early October from GD2 (Supplementary Table S1 ). The samples were originally collected from Devon in the southwest of England (Highfield et al., 2009) . Twenty asymptomatic bees were pooled for each sample before RNA extraction. RNA extractions were prepared as in Highfield et al. (2009) followed by a complementary DNA amplification step before sequencing.
Search related documents:
Co phrase search for related documents- dna amplification and sequence dna amplification step: 1
- dna amplification and Supplementary table: 1, 2, 3, 4, 5
- dna amplification step and sequence dna amplification step: 1
- Hi seq and pair end: 1
- Hi seq and pair end sequencing: 1
- Hi seq and Supplementary table: 1
- pair end and rna extraction: 1
- pair end and Supplementary table: 1
- pair end sequencing and Supplementary table: 1
- rna extraction and sample pool: 1, 2, 3, 4
- rna extraction and Supplementary table: 1
Co phrase search for related documents, hyperlinks ordered by date