Diversity in a honey bee pathogen: first report of a third master variant of the Deformed Wing Virus quasispecies

Mordecai, Gideon J; Wilfert, Lena; Martin, Stephen J; Jones, Ian M; Schroeder, Declan C

Sequencing and assembly Total RNA was sequenced without an amplification step. Illumina (San Diego, CA, USA) Hi-seq (2 × 100) pair-end sequencing was carried out by TGAC (The Genome Analysis Centre) and at the University of Exeter on four samples from Highfield et al. (2009) ; Late June, Early October and Late October from GD1, and early October from GD2 (Supplementary Table S1 ). The samples were originally collected from Devon in the southwest of England (Highfield et al., 2009) . Twenty asymptomatic bees were pooled for each sample before RNA extraction. RNA extractions were prepared as in Highfield et al. (2009) followed by a complementary DNA amplification step before sequencing.