Title: The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells Document date: 1989_5_1
ID: kjid2e3q_17
Snippet: 60-ram dishes were seeded with 2 x 106 cells, 24-72 h later the cells were washed with PBS and pulse-labeled with 1 ml of labeling medium supplemented with 2 mM glutamine, 250 #Ci/ml [35S]methionine exactly as previously described (41) . For chase incubations, the medium also contained 10 #l/ml of antiserum (41) . After the labeling and chase periods, the medium was removed, centrifuged, and stored on ice or at -20°C until further treated. Cells.....
Document: 60-ram dishes were seeded with 2 x 106 cells, 24-72 h later the cells were washed with PBS and pulse-labeled with 1 ml of labeling medium supplemented with 2 mM glutamine, 250 #Ci/ml [35S]methionine exactly as previously described (41) . For chase incubations, the medium also contained 10 #l/ml of antiserum (41) . After the labeling and chase periods, the medium was removed, centrifuged, and stored on ice or at -20°C until further treated. Cells were harvested (41), the suspension centrifuged, and the pellet lysed by vortexing in lysis buffer. Nuclei were removed by centrifugation (41) and the postnuclear supernatants were stored on ice or at -20°C until treated with antisera.
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