Selected article for: "Coomassie Blue staining and recombinant protein"
Author: Ha, Cam T.; Wu, Julie A.; Irmak, Ster; Lisboa, Felipe A.; Dizon, Anne M.; Warren, James W.; Ergun, Suleyman; Dveksler, Gabriela S.
Title: Human Pregnancy Specific Beta-1-Glycoprotein 1 (PSG1) Has a Potential Role in Placental Vascular Morphogenesis
  • Document date: 2010_7_1
    • ID: k2vbgqk7_30
    Snippet: The human PSG family has 11 members, and PSG1 is the most abundant family member [29] . We generated recombinant PSG1-FLAG, which consists of the leader peptide, followed by the N, A2, and B2 domains of PSG1 and then a FLAG, V5, and six His tags. The recombinant protein has an approximate molecular mass of 55 kDa and was harvested from the supernatant of stably transfected CHO cells, as described in Materials and Methods. Recombinant PSG1-FLAG pr.....
    Document: The human PSG family has 11 members, and PSG1 is the most abundant family member [29] . We generated recombinant PSG1-FLAG, which consists of the leader peptide, followed by the N, A2, and B2 domains of PSG1 and then a FLAG, V5, and six His tags. The recombinant protein has an approximate molecular mass of 55 kDa and was harvested from the supernatant of stably transfected CHO cells, as described in Materials and Methods. Recombinant PSG1-FLAG protein was sequentially purified on a HisTrap column followed by an anti-FLAG affinity column. These two purification steps rendered a glycoprotein that was more than 90% pure based on Coomassie blue staining (Fig. 1, lane 1) .

    Search related documents:
    Co phrase search for related documents
    • affinity column and molecular mass: 1, 2
    • affinity column and purification step: 1, 2, 3, 4
    • affinity column and recombinant protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • blue staining and Methods material: 1
    • blue staining and recombinant protein: 1, 2, 3, 4, 5, 6
    • CHO cell and purification step: 1, 2
    • CHO cell and recombinant protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • CHO cell and stably transfected CHO cell: 1, 2, 3
    • HisTrap column and purification step: 1, 2, 3
    • HisTrap column and recombinant protein: 1, 2, 3
    • human PSG family and PSG family: 1, 2, 3
    • Methods material and recombinant protein: 1
    • molecular mass and recombinant protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • purification step and recombinant protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13