Author: Rasmy, Hanaa; Mikhael, Nancy; Ismail, Somaia
Title: Interleukin-18 expression and the response to treatment in patients with psoriasis Document date: 2011_9_2
ID: jppdl104_15
Snippet: Total RNA was extracted using QIA amp RNA extraction kit provided by QIAGEN worldwide companies. The concentration and purity of RNA was determined by measuring its absorbance at 260 nm (A 260 ) and 280 nm (A 280 ) in a UV visible spectrophotometer. Total RNA (2 μg) was reversetranscribed to cDNA with High-Capacity cDNA Archive kit provided by Applied Biosystems. cDNAs were stored at -20°C until real-time quantitative PCR was performed......
Document: Total RNA was extracted using QIA amp RNA extraction kit provided by QIAGEN worldwide companies. The concentration and purity of RNA was determined by measuring its absorbance at 260 nm (A 260 ) and 280 nm (A 280 ) in a UV visible spectrophotometer. Total RNA (2 μg) was reversetranscribed to cDNA with High-Capacity cDNA Archive kit provided by Applied Biosystems. cDNAs were stored at -20°C until real-time quantitative PCR was performed.
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