Author: Hui-Yuen, Joyce; McAllister, Shane; Koganti, Siva; Hill, Erik; Bhaduri-McIntosh, Sumita
Title: Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines Document date: 2011_11_8
ID: kss857n1_8
Snippet: 1. Add FK506 (AG Scientific) to the cell suspension from 2.6 to a final concentration of 20 nM. Place flask in CO 2 incubator at 37°C for one hour. 2. Rapidly thaw an aliquot of EBV. Remove flask from incubator and add EBV to the cells at 1/10 dilution. Typically, this dilution provides an MOI of 50-100. Swirl flask to mix and place it upright in CO 2 incubator at 37°C......
Document: 1. Add FK506 (AG Scientific) to the cell suspension from 2.6 to a final concentration of 20 nM. Place flask in CO 2 incubator at 37°C for one hour. 2. Rapidly thaw an aliquot of EBV. Remove flask from incubator and add EBV to the cells at 1/10 dilution. Typically, this dilution provides an MOI of 50-100. Swirl flask to mix and place it upright in CO 2 incubator at 37°C.
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