Selected article for: "cell line and International license"
Author: Vojo Deretic; Graham S Timmins
Title: Azithromycin and ciprofloxacin have a chloroquine-like effect on respiratory epithelial cells
  • Document date: 2020_3_31
    • ID: i4ijuk36_20
    Snippet: . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.008631 doi: bioRxiv preprint Methods Cells, tissue culture and chemicals. IB3-1 is a bronchial epithelial cell line derived from a CF patient with a ΔF508/W1282X CFTR mutant genotype 65 . S9 is a derivative of IB3-1 corrected for chloride conductance by s.....
    Document: . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.008631 doi: bioRxiv preprint Methods Cells, tissue culture and chemicals. IB3-1 is a bronchial epithelial cell line derived from a CF patient with a ΔF508/W1282X CFTR mutant genotype 65 . S9 is a derivative of IB3-1 corrected for chloride conductance by stable transfection with a functional CFTR. The cells were maintained in LHC-8 Media (Biosource Int., MD). The CF cell line CFBE41ō (ΔF508/ΔF508) and normal human bronchial epithelial cells16HBEō (from D. Gruenert) were maintained in complete MEMmedia (GibcoBRL, Life Technologies, MD). The normal human tracheal cell line 9HTEō, transfected with pCEP-R or pCEP were from P. Davis, and were maintained in complete DMEM (GibcoBRL, Life Technologies, MD). Primary bronchial epithelial cells were from Clonetics (NHBE) or from P. Karp (CF lung transplants) and were maintained in supplemented BEMB (Lonza, MD). AZT was from Fluke (Switzerland) and CPX-HCL was from Sigma (St. Louis, MO).

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