Author: Xin Zeng; Lingfang Li; Jing Lin; Xinlei Li; Bin Liu; Yang Kong; Shunze Zeng; Jianhua Du; Huahong Xiao; Tao Zhang; Shelin Zhang; Jianghai Liu
Title: Blocking antibodies against SARS-CoV-2 RBD isolated from a phage display antibody library using a competitive biopanning strategy Document date: 2020_4_20
ID: 4w6caxwu_17
Snippet: VH and VL of the positive phage were subcloned respectively into the pFUSEss-CHIg-hG1 and pFUSEss-CLIg-hK (Invitrogen). Antibodies were transiently expressed in FreeStyleâ„¢ HEK 293-F cells (Life Technologies) using 293fectin transfection reagent according to manufacturer's instructions. After transfection, cells were grown in the serum-free medium for an additional 5 days. The supernatant was collected and purified on a MabSelect Protein A colum.....
Document: VH and VL of the positive phage were subcloned respectively into the pFUSEss-CHIg-hG1 and pFUSEss-CLIg-hK (Invitrogen). Antibodies were transiently expressed in FreeStyle™ HEK 293-F cells (Life Technologies) using 293fectin transfection reagent according to manufacturer's instructions. After transfection, cells were grown in the serum-free medium for an additional 5 days. The supernatant was collected and purified on a MabSelect Protein A column (GE healthcare). Eluted IgG was dialyzed against PBS and stored at -80°C.
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