Author: Hernandez, Nicholas; Melki, Isabelle; Jing, Huie; Habib, Tanwir; Huang, Susie S.Y.; Danielson, Jeffrey; Kula, Tomasz; Drutman, Scott; Belkaya, Serkan; Rattina, Vimel; Lorenzo-Diaz, Lazaro; Boulai, Anais; Rose, Yoann; Kitabayashi, Naoki; Rodero, Mathieu P.; Dumaine, Cecile; Blanche, Stéphane; Lebras, Marie-Noëlle; Leung, Man Chun; Mathew, Lisa Sara; Boisson, Bertrand; Zhang, Shen-Ying; Boisson-Dupuis, Stephanie; Giliani, Silvia; Chaussabel, Damien; Notarangelo, Luigi D.; Elledge, Stephen J.; Ciancanelli, Michael J.; Abel, Laurent; Zhang, Qian; Marr, Nico; Crow, Yanick J.; Su, Helen C.; Casanova, Jean-Laurent
Title: Life-threatening influenza pneumonitis in a child with inherited IRF9 deficiency Document date: 2018_10_1
ID: jqv0lyfx_35
Snippet: PBMCs were isolated by Ficoll-Paque density gradient (Lymphoprep; Proteogenix) from the blood of the patient, the mother, and a healthy donor. SV40-immortalized dermal fibroblasts, U2A, Madin-Darby canine kidney, and Vero E6 cells were maintained in DMEM supplemented with 10% FBS. B-LCLs were grown in RPMI 1640 medium supplemented with 10% FBS. Stably transfected dermal fibroblasts were obtained by transfecting pTRI PIRF9iresRFP using Lipofectami.....
Document: PBMCs were isolated by Ficoll-Paque density gradient (Lymphoprep; Proteogenix) from the blood of the patient, the mother, and a healthy donor. SV40-immortalized dermal fibroblasts, U2A, Madin-Darby canine kidney, and Vero E6 cells were maintained in DMEM supplemented with 10% FBS. B-LCLs were grown in RPMI 1640 medium supplemented with 10% FBS. Stably transfected dermal fibroblasts were obtained by transfecting pTRI PIRF9iresRFP using Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol. Stable transfectants were selected by puromycin treatment (0.3 μg/ml) and subsequent fluorescence-activated cell sorting for RFP-expressing cells yielding >80% RFP + fibroblasts.
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