Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing Document date: 2012_11_30
ID: jiy4cp4n_12
Snippet: The branched or bDNA signal amplification system consists of a series of hybridization steps resulting in a "sandwich" complex of probes and target sequence with a branched structure [11] . In this technique, multiple targetspecific probes are used to capture the nucleic acid onto the surface of a microwell plate. The initial step in a bDNA assay is to ensure that viral particles have been disrupted and that viral RNA is present for analysis. Tar.....
Document: The branched or bDNA signal amplification system consists of a series of hybridization steps resulting in a "sandwich" complex of probes and target sequence with a branched structure [11] . In this technique, multiple targetspecific probes are used to capture the nucleic acid onto the surface of a microwell plate. The initial step in a bDNA assay is to ensure that viral particles have been disrupted and that viral RNA is present for analysis. Target-specific oligonucleotides are then hybridized to the target nucleic acid, and other regions hybridize to multiple bDNA amplifier molecules that create a branched structure. Finally, the bDNA signal is the chemiluminiscent product of the reaction [11] . The signal in the bDNA assay is proportional to the number of labeled probes bDNA assays for the quantitation of hepatitis C virus (HCV) RNA, hepatitis B virus (HBV) DNA and human immunodeficiency virus type 1 (HIV-1) RNA are commercially available (Bayer HealthCare, Diagnostics Division, Tarrytown, N.Y.).
Search related documents:
Co phrase search for related documents- amplification system and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- amplification system and nucleic acid target: 1, 2
- amplification system and signal amplification system: 1, 2
- amplification system and virus type: 1, 2
Co phrase search for related documents, hyperlinks ordered by date