Author: Mandapathil, M.; H.Beier, U.; Graefe, H.; Kröger, B.; Hedderich, J.; Maune, S.; Meyer, J. E
Title: Differential chemokine expression patterns in tonsillar disease Document date: 2018_8_23
ID: jirwy2kj_16
Snippet: Quality assessment of the isolated RNA was made by agarose gel electrophoresis exhibiting non-fragmented RNA with sufficient quantity for reverse transcription and subsequent processing. After RT-PCR procedures, samples were processed by agarose gel electrophoresis, and the amplicons of the chemokines were measured and adjusted to the relative G3PDH signal strength, comparative anal-ysis was initiated. All data followed normal distribution and ar.....
Document: Quality assessment of the isolated RNA was made by agarose gel electrophoresis exhibiting non-fragmented RNA with sufficient quantity for reverse transcription and subsequent processing. After RT-PCR procedures, samples were processed by agarose gel electrophoresis, and the amplicons of the chemokines were measured and adjusted to the relative G3PDH signal strength, comparative anal-ysis was initiated. All data followed normal distribution and are displayed as mean ± SD in the following. Four cohorts were examined: patients with tonsillar hyperplasia (n = 21), adenoids (n = 25), chronic tonsillitis (n = 16) and chronic tonsillitis with histological proof of acute inflammation (further in the text referred to as "acute tonsillitis") (n = 27).
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