Author: Zhao, Jingxian; Zhao, Jincun; Fett, Craig; Trandem, Kathryn; Fleming, Erica; Perlman, Stanley
Title: IFN-?– and IL-10–expressing virus epitope-specific Foxp3(+) T reg cells in the central nervous system during encephalomyelitis Document date: 2011_8_1
ID: k751ryv4_35
Snippet: were captured using a mouse IFN-î§ secretion assay (Miltenyi Biotec) as previously described (Pewe et al., 2004) . IFN-î§-expressing CD4 T cells were sorted with a FACSDiva (BD). RNA was isolated using an RNeasy Mini kit (Invitrogen) and used for cDNA synthesis. Vî¢8-specific PCR products were synthesized because Vî¢8 is the most commonly used Vî¢ element in the M133-specific response (Haring et al., 2001) . PCR products were cloned into pla.....
Document: were captured using a mouse IFN-î§ secretion assay (Miltenyi Biotec) as previously described (Pewe et al., 2004) . IFN-î§-expressing CD4 T cells were sorted with a FACSDiva (BD). RNA was isolated using an RNeasy Mini kit (Invitrogen) and used for cDNA synthesis. Vî¢8-specific PCR products were synthesized because Vî¢8 is the most commonly used Vî¢ element in the M133-specific response (Haring et al., 2001) . PCR products were cloned into plasmid vector pCR2.1-TOPO (Invitrogen) and sequenced.
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