Selected article for: "antibody neutralization and pseudovirus neutralization"

Author: Xin Zeng; Lingfang Li; Jing Lin; Xinlei Li; Bin Liu; Yang Kong; Shunze Zeng; Jianhua Du; Huahong Xiao; Tao Zhang; Shelin Zhang; Jianghai Liu
Title: Blocking antibodies against SARS-CoV-2 RBD isolated from a phage display antibody library using a competitive biopanning strategy
  • Document date: 2020_4_20
  • ID: 4w6caxwu_30
    Snippet: As a positive control, the recombinant ACE2-hFc (100µg/ml) totally inhibited the infection of ACE2-overexpressing Hela monoclonal cells with SARS-CoV-2 pseudovirus. The antibody rRBD-15 showed a significant neutralization activity against the SARS-CoV-2 pseudovirus with IC50 values of 12.2nM. However, the antibody rRBD-16 had no neutralization effect of the pseudovirus and there were no significant differences between the highest concentration a.....
    Document: As a positive control, the recombinant ACE2-hFc (100µg/ml) totally inhibited the infection of ACE2-overexpressing Hela monoclonal cells with SARS-CoV-2 pseudovirus. The antibody rRBD-15 showed a significant neutralization activity against the SARS-CoV-2 pseudovirus with IC50 values of 12.2nM. However, the antibody rRBD-16 had no neutralization effect of the pseudovirus and there were no significant differences between the highest concentration antibody group and the blank group without antibody addition.

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