Selected article for: "RNA virus and virus detection"
Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing
  • Document date: 2012_11_30
    • ID: jiy4cp4n_29
    Snippet: There are two methods included in this group: nucleic acid sequence-based amplification (NASBA) and transcription-mediated amplification (TMA). These are isothermal RNA amplification methods modeled after retroviral replication [20, 21] . In both methods, the RNA target is reverse transcribed into cDNA and then RNA copies are synthesized with a RNA polymerase. Transcriptionbased amplification systems have several characteristics such as no requir.....
    Document: There are two methods included in this group: nucleic acid sequence-based amplification (NASBA) and transcription-mediated amplification (TMA). These are isothermal RNA amplification methods modeled after retroviral replication [20, 21] . In both methods, the RNA target is reverse transcribed into cDNA and then RNA copies are synthesized with a RNA polymerase. Transcriptionbased amplification systems have several characteristics such as no requirement for a thermal cycler, rapid kinetics and a single-stranded RNA product that does not require denaturation prior to detection. Gen-Probe has developed TMA-based assays for detection of HCV and HIV-1, while NASBA-based kits (bioMérieux) have developed commercially available kits for the detection and quantitation of HIV-1 RNA and CMV RNA and detection of enterovirus and respiratory syncytial virus RNA.

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