Selected article for: "cdna strand synthesis and synthesis kit"
Author: Mandapathil, M.; H.Beier, U.; Graefe, H.; Kröger, B.; Hedderich, J.; Maune, S.; Meyer, J. E
Title: Differential chemokine expression patterns in tonsillar disease
  • Document date: 2018_8_23
    • ID: jirwy2kj_6
    Snippet: Reverse transcription 1.0 µg RNA was heat-denatured (65°C, 10 min), chilled on ice, and subjected to random hexadeoxynucleotide primed reverse transcription using the first strand cDNA synthesis kit (Pharmacia, Freiburg, Germany). Reverse transcription (final volume 15 µl) was conducted at 37°C for 60 min in the presence of 0.2 µM of random hexanucleotide primer and 40 U RNase inhibitor (RNAsin, Gibco, Germany). Following synthesis of the co.....
    Document: Reverse transcription 1.0 µg RNA was heat-denatured (65°C, 10 min), chilled on ice, and subjected to random hexadeoxynucleotide primed reverse transcription using the first strand cDNA synthesis kit (Pharmacia, Freiburg, Germany). Reverse transcription (final volume 15 µl) was conducted at 37°C for 60 min in the presence of 0.2 µM of random hexanucleotide primer and 40 U RNase inhibitor (RNAsin, Gibco, Germany). Following synthesis of the completed first strand cDNA the resulting RNA-cDNA double-stranded helix was heat-denatured (95°C, 5 min) to provide cDNA as a template for polymerisation.

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