Author: Jennifer A. Doudna
Title: Blueprint for a Pop-up SARS-CoV-2 Testing Lab Document date: 2020_4_12
ID: modtthxx_55
Snippet: To lower the overall cost of our assay per test, and to limit reagent use in the face of supply chain shortages, we sought to reduce the quantity of reagents used per RNA extraction and RT-qPCR assay. After pilot experiments demonstrated that the test performed robustly after such a reduction, we proceeded to validate our workflow at half-reaction sizes (see supplementary file, Semi-Manual SOP, for exact volumes). In the 13 . CC-BY-ND 4.0 Interna.....
Document: To lower the overall cost of our assay per test, and to limit reagent use in the face of supply chain shortages, we sought to reduce the quantity of reagents used per RNA extraction and RT-qPCR assay. After pilot experiments demonstrated that the test performed robustly after such a reduction, we proceeded to validate our workflow at half-reaction sizes (see supplementary file, Semi-Manual SOP, for exact volumes). In the 13 . CC-BY-ND 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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