Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage Document date: 2017_9_29
ID: k4gtl2o7_1
Snippet: Non-standard events during the elongation phase of transcription can either enrich gene expression or contribute to erroneous and wasteful expression. An example of the former is selection for reverse transcriptase-mediated multiple alternative base substitutions to lead to pathogen surface variability to evade host defenses (1) . A different type of productive non-standard polymerase action involves realignment of the template:product hybrid at .....
Document: Non-standard events during the elongation phase of transcription can either enrich gene expression or contribute to erroneous and wasteful expression. An example of the former is selection for reverse transcriptase-mediated multiple alternative base substitutions to lead to pathogen surface variability to evade host defenses (1) . A different type of productive non-standard polymerase action involves realignment of the template:product hybrid at a slippage-prone sequence to yield product with extra or fewer base(s) than present in the corresponding template sequence (2) . This has been studied with DNA-dependent DNA polymerases, DNA-dependent RNA polymerases, RNA-dependent RNA polymerases and RNA-dependent DNA polymerases (reverse transcriptases, RTs).
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