Selected article for: "luciferase expression and Renilla luciferase plasmid"
Author: Hernandez, Nicholas; Melki, Isabelle; Jing, Huie; Habib, Tanwir; Huang, Susie S.Y.; Danielson, Jeffrey; Kula, Tomasz; Drutman, Scott; Belkaya, Serkan; Rattina, Vimel; Lorenzo-Diaz, Lazaro; Boulai, Anais; Rose, Yoann; Kitabayashi, Naoki; Rodero, Mathieu P.; Dumaine, Cecile; Blanche, Stéphane; Lebras, Marie-Noëlle; Leung, Man Chun; Mathew, Lisa Sara; Boisson, Bertrand; Zhang, Shen-Ying; Boisson-Dupuis, Stephanie; Giliani, Silvia; Chaussabel, Damien; Notarangelo, Luigi D.; Elledge, Stephen J.; Ciancanelli, Michael J.; Abel, Laurent; Zhang, Qian; Marr, Nico; Crow, Yanick J.; Su, Helen C.; Casanova, Jean-Laurent
Title: Life-threatening influenza pneumonitis in a child with inherited IRF9 deficiency
  • Document date: 2018_10_1
    • ID: jqv0lyfx_43
    Snippet: Reporter assays U2A cells were transfected with the indicated expression plasmids, firefly luciferase plasmids under the control of ISRE or GAS promoters, and a constitutively expressing Renilla firefly luciferase plasmid for normalization. 24 h after transfection, the cells were stimulated with IFN-α2b, -β, or -γ for 18 h or were left unstimulated. Luciferase levels were measured using Dual-Glo reagent according to the manufacturer's protocol.....
    Document: Reporter assays U2A cells were transfected with the indicated expression plasmids, firefly luciferase plasmids under the control of ISRE or GAS promoters, and a constitutively expressing Renilla firefly luciferase plasmid for normalization. 24 h after transfection, the cells were stimulated with IFN-α2b, -β, or -γ for 18 h or were left unstimulated. Luciferase levels were measured using Dual-Glo reagent according to the manufacturer's protocol (Promega). Firefly luciferase values are normalized to Renilla luciferase values, and fold induction is expressed relative to unstimulated samples.

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