Author: Weishan Huang; Sabrina Solouki; Chavez Carter; Song-Guo Zheng; Avery August
Title: Beyond type 1 regulatory T cells: co-expression of LAG3 and CD49b in IL-10-producing T cell lineages Document date: 2018_6_30
ID: ivyp5cgz_10
Snippet: Center, Long Island, NY) using gradient separation in Ficoll-Paque PLUS (GE Healthcare). PBMC 161 were cultured in full RPMI-1640 medium for 30 mins in 37ºC, then non-adherent cells were used 162 to enrich CD4 + T cells using anti-human CD4 microbeads (Miltenyl Biotec, San Diego, CA) or 163 CD8 + T cells using a human CD8 isolation kit (BioLegend). Adherent cells were treated with 164.....
Document: Center, Long Island, NY) using gradient separation in Ficoll-Paque PLUS (GE Healthcare). PBMC 161 were cultured in full RPMI-1640 medium for 30 mins in 37ºC, then non-adherent cells were used 162 to enrich CD4 + T cells using anti-human CD4 microbeads (Miltenyl Biotec, San Diego, CA) or 163 CD8 + T cells using a human CD8 isolation kit (BioLegend). Adherent cells were treated with 164
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