Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing Document date: 2012_11_30
ID: jiy4cp4n_23
Snippet: This modality of PCR increases both the sensitivity and specificity [15] . This technique uses two pairs of amplification primers and two rounds of PCR. In the first round, it uses one primer pair for 15 to 30 cycles. The product of the first round of amplification is submitted to a second round of amplification with the second pair of primers. The major disadvantage of nested PCR is the high rates of contamination......
Document: This modality of PCR increases both the sensitivity and specificity [15] . This technique uses two pairs of amplification primers and two rounds of PCR. In the first round, it uses one primer pair for 15 to 30 cycles. The product of the first round of amplification is submitted to a second round of amplification with the second pair of primers. The major disadvantage of nested PCR is the high rates of contamination.
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