Selected article for: "high quality and HT sequencing"

Author: Ladner, Jason T.; Beitzel, Brett; Chain, Patrick S. G.; Davenport, Matthew G.; Donaldson, Eric; Frieman, Matthew; Kugelman, Jeffrey; Kuhn, Jens H.; O’Rear, Jules; Sabeti, Pardis C.; Wentworth, David E.; Wiley, Michael R.; Yu, Guo-Yun; Sozhamannan, Shanmuga; Bradburne, Christopher; Palacios, Gustavo
Title: Standards for Sequencing Viral Genomes in the Era of High-Throughput Sequencing
  • Document date: 2014_6_17
  • ID: kqcx7lrq_8
    Snippet: High quality (HQ). Genomes should be considered high quality if no gaps remain (i.e., a single contig per genome/segment), even if one or more ORFs remain incomplete due to missing sequence at the ends of segments. An HQ genome can often be achieved with modest levels of HT sequencing coverage (~15 to 30Ï«) or through Sanger-mediated gap resolution of an SD......
    Document: High quality (HQ). Genomes should be considered high quality if no gaps remain (i.e., a single contig per genome/segment), even if one or more ORFs remain incomplete due to missing sequence at the ends of segments. An HQ genome can often be achieved with modest levels of HT sequencing coverage (~15 to 30Ï«) or through Sanger-mediated gap resolution of an SD.

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