Author: Castrignano, Silvana Beres; Nagasse-Sugahara, Teresa Keico
Title: The metagenomic approach and causality in virology Document date: 2015_4_1
ID: ivu4erpq_16
Snippet: Viral metagenomics has been having a great impact on the discovery of new viruses because it enables the detection of all viral genomes present in a given sample independently from antisera tests, from previous knowledge of the viral genome (unlike other molecular biology techniques such as polymerase chain reaction, microarray, and in situ hybridization), and from cell culture isolation. 2, 16, 27 However, the causal association between a virus .....
Document: Viral metagenomics has been having a great impact on the discovery of new viruses because it enables the detection of all viral genomes present in a given sample independently from antisera tests, from previous knowledge of the viral genome (unlike other molecular biology techniques such as polymerase chain reaction, microarray, and in situ hybridization), and from cell culture isolation. 2, 16, 27 However, the causal association between a virus and a disease in humans and other animals still depends on a set of clinical, epidemiological, and laboratory investigations; on the use of strict criteria to associate these elements, such as those in Koch's postulates and their adapted versions; and on common sense during data analysis. 9, 10, 12, 13, 16, 19
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