Author: Hernes, S. S.; Quarsten, H.; Hagen, E.; Lyngroth, A. L.; Pripp, A. H.; Bjorvatn, B.; Bakke, P. S.
Title: Swabbing for respiratory viral infections in older patients: a comparison of rayon and nylon flocked swabs Document date: 2010_9_18
ID: ivu632j2_19
Snippet: Specimens were kept at room temperature until nucleic acid extraction, which usually took place within 24 h and always within 48 h. PCR analyses were performed within the next 24 h or else the nucleic acid eluates were stored at −70°C until testing. Using monoplex (RSV and human metapneumovirus) or multiplex (influenza A/B, adenovirus/internal control and parainfluenza virus 1-4) PCR methods, the specimens were examined for, in total, nine dif.....
Document: Specimens were kept at room temperature until nucleic acid extraction, which usually took place within 24 h and always within 48 h. PCR analyses were performed within the next 24 h or else the nucleic acid eluates were stored at −70°C until testing. Using monoplex (RSV and human metapneumovirus) or multiplex (influenza A/B, adenovirus/internal control and parainfluenza virus 1-4) PCR methods, the specimens were examined for, in total, nine different respiratory viruses (Table 2) .
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