Author: Mauthe, Mario; Langereis, Martijn; Jung, Jennifer; Zhou, Xingdong; Jones, Alex; Omta, Wienand; Tooze, Sharon A.; Stork, Björn; Paludan, Søren Riis; Ahola, Tero; Egan, Dave; Behrends, Christian; Mokry, Michal; de Haan, Cornelis; van Kuppeveld, Frank; Reggiori, Fulvio
Title: An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication Document date: 2016_8_29
ID: iuqa0yrw_44
Snippet: Virus stocks were generated and propagated as described previously. Fluc-HSV-1 (Paludan virus stock collection), SFV-Rluc was produced from the infectious clone SFV-RlucH2 and propagated in BHK cells (Pohjala et al., 2011) , MHV-2aFLS was propagated on LR7 mouse fibroblast cells (de Haan et al., 2003) , Fluc-VaV was propagated in African green monkey kidney BSC-40 cells (Rodriguez et al., 1988; de Vries et al., 2011) , and IAV-WSN luciferase pseu.....
Document: Virus stocks were generated and propagated as described previously. Fluc-HSV-1 (Paludan virus stock collection), SFV-Rluc was produced from the infectious clone SFV-RlucH2 and propagated in BHK cells (Pohjala et al., 2011) , MHV-2aFLS was propagated on LR7 mouse fibroblast cells (de Haan et al., 2003) , Fluc-VaV was propagated in African green monkey kidney BSC-40 cells (Rodriguez et al., 1988; de Vries et al., 2011) , and IAV-WSN luciferase pseudovirus was produced in a MDCK cell line that stably expresses the HA of IAV-WSN (König et al., 2010; de Vries et al., 2011) . EMCV, EMCV-Zn wild type (Hato et al., 2007) , and Rluc-EMCV (Lanke et al., 2009) were generated by transfecting runoff transcript RNA from the BamHI linearized pM16.1 infectious clone into BHK21 cells. The CVB3 and RLuc-CVB3 (Wessels et al., 2005) viruses were generated by transfecting runoff transcript RNA from the SalI linearized p53CVB3/T7 infectious clone into BGM cells. CVB3, EV68 (Ulferts et al., 2013) , EV-71, and CVA21 (van der Schaar et al., 2013) viruses were propagated in HeLa-R19 cells, whereas EMCV was propagated in BHK21 cells.
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