Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head

Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min

Animal ONFH model and treatment protocol. A total of 50 New Zealand rabbits (age, 2-3 months) were used to established ONFH models according to previously reported protocols (25) . In brief, one injection of lipopolysaccharide (LPS; 10 µg/kg; Sigma-Aldrich) was administered intravenously at day 1, then three injections of methylprednisolone (MPS; 20 mg/kg; Pfizer, Inc., New York, NY, USA) were administered intramuscularly at days 2, 3 and 4. Six rabbits died of the inductive protocol after the injection. ONFH was confirmed using magnetic resonance imaging (MRI; GE Healthcare, Chicago, IL, USA) at 6 weeks after the last injection of MPS. The stage of ONFH was evaluated respectively by two experienced radiologists in a blinded fashion. Then the rabbits with early-stage ONFH were randomly divided into four groups: i) Group I (n=11) did not receive any therapy and served as controls; ii) Group II (n=11) only received core decompression of the femoral head; iii) Group III (n=11) received core decompression and normal ASC transplantation; and iv) Group IV (n=11) received core decompression and DMOG-treated ASC transplantation.