Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head

Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min

Bone regeneration in the necrotic area of each group was initially analyzed using micro-CT scanning. Bone microarchitecture of each group was reconstructed in three dimensions for presentation ( Fig. 2A) . The samples of Group I, which received no treatment, showed few destroyed trabeculae in the necrotic area of the femoral head. In Group II, which received core decompression, the trabeculae in the necrotic area was thin and sparse. In Group III, which received core decompression and transplantation of normal ASCs, the trabeculae in the necrotic area was more intact. In Group IV, which received core decompression and transplantation of DMOG treated ASCs, the trabeculae in the necrotic area appeared intact and well distributed. Quantitative analysis indicated nearly no new bone formation in the necrotic area of Group I (Fig. 2B) . By contrast, ~25.2±2.8% of the necrotic area was regenerated in Group II, 45.5±3.4% in Group III and 58.8±7.4% in Group IV. The BMD of the new bone in the necrotic area of Group IV was also significantly higher than that of other three groups (P<0.05).