Author: Ha, Cam T.; Wu, Julie A.; Irmak, Ster; Lisboa, Felipe A.; Dizon, Anne M.; Warren, James W.; Ergun, Suleyman; Dveksler, Gabriela S.
Title: Human Pregnancy Specific Beta-1-Glycoprotein 1 (PSG1) Has a Potential Role in Placental Vascular Morphogenesis Document date: 2010_7_1
ID: k2vbgqk7_45
Snippet: Two other members of the VEGF family, VEGFC and PGF, have been shown to be important for normal placental development [35] [36] [37] [38] . We tested whether PSG1 induced VEGFC and PGF in different cell types. Expression of PGF has previously been reported in trophoblasts and endothelial cells [39] . Our results showed that PSG1 did not induce PGF in HEEC (Fig. 6A) , HUVECs (Fig. 6B) , and UtMVECs (data not shown). The choriocarcinoma cell line B.....
Document: Two other members of the VEGF family, VEGFC and PGF, have been shown to be important for normal placental development [35] [36] [37] [38] . We tested whether PSG1 induced VEGFC and PGF in different cell types. Expression of PGF has previously been reported in trophoblasts and endothelial cells [39] . Our results showed that PSG1 did not induce PGF in HEEC (Fig. 6A) , HUVECs (Fig. 6B) , and UtMVECs (data not shown). The choriocarcinoma cell line BeWo secreted high basal levels of PGF, and no significant difference in control protein and PSG1-treated wells was found (Fig. 6C) . On the other hand, the basal level of PGF secretion by HTR-8 SVneo cells was low-at the level of the lowest ELISA standard-but as for BeWo cells, no induction by PSG1 could be detected (data not shown).
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