Author: Lam, Yun W.; Evans, Vanessa C.; Heesom, Kate J.; Lamond, Angus I.; Matthews, David A.
Title: Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells Document date: 2009_10_7
ID: jgxbpy4j_6
Snippet: Cells and Viruses-HeLa cells were grown in Dulbecco's modified Eagle's medium Ï© Glutamax (Invitrogen) supplemented with 10% fetal calf serum, 100 g/ml streptomycin, and 100 IU/ml penicillin. Human adenovirus type 5 (Ad5) was propagated as described previously (33) and purified using a ViraBind adenovirus purification kit (Cellbiolabs). The HeLa cell line stably expressing GFP-tagged ribosomal protein RPL27 has been reported elsewhere (34) . For .....
Document: Cells and Viruses-HeLa cells were grown in Dulbecco's modified Eagle's medium Ï© Glutamax (Invitrogen) supplemented with 10% fetal calf serum, 100 g/ml streptomycin, and 100 IU/ml penicillin. Human adenovirus type 5 (Ad5) was propagated as described previously (33) and purified using a ViraBind adenovirus purification kit (Cellbiolabs). The HeLa cell line stably expressing GFP-tagged ribosomal protein RPL27 has been reported elsewhere (34) . For microscopy, HeLa cells were grown on glass coverslips in 6-well dishes.
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