Selected article for: "RNA stem loop structure and stem loop"
Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage
  • Document date: 2017_9_29
    • ID: k4gtl2o7_24
    Snippet: To assess potential intermolecular stem-loop structure stimulatory action, we used an RNA antisense (anti-3 stem) complementary to the 10 nt sequence 5 adjacent to the A7 motif in the MUTsl-A7' RNA construct (Figure 2A ). The results, Figure 2B , show a strong effect of the antisense RNA on slippage directionality and efficiency. This antisense result with 'MUTsl-A7' is similar to the RT realignment without antisense with the 'wtSL-A7' construct .....
    Document: To assess potential intermolecular stem-loop structure stimulatory action, we used an RNA antisense (anti-3 stem) complementary to the 10 nt sequence 5 adjacent to the A7 motif in the MUTsl-A7' RNA construct (Figure 2A ). The results, Figure 2B , show a strong effect of the antisense RNA on slippage directionality and efficiency. This antisense result with 'MUTsl-A7' is similar to the RT realignment without antisense with the 'wtSL-A7' construct 1-4) .

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