Author: Lam, Yun W.; Evans, Vanessa C.; Heesom, Kate J.; Lamond, Angus I.; Matthews, David A.
Title: Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells Document date: 2009_10_7
ID: jgxbpy4j_12
Snippet: Mass spectra were recorded in positive ion reflector mode on an Applied Biosystems 4700 MALDI mass spectrometer. The instrument was calibrated before each run using the 4700 Calibration Mix 1 (Applied Biosystems), and in addition, individual MS spectra were internally calibrated using tryptic autolytic peaks (if present). MSMS analysis was calibrated on the fragmentation of Glu-fibrinopeptide. For MSMS analysis, the top five most intense, non-try.....
Document: Mass spectra were recorded in positive ion reflector mode on an Applied Biosystems 4700 MALDI mass spectrometer. The instrument was calibrated before each run using the 4700 Calibration Mix 1 (Applied Biosystems), and in addition, individual MS spectra were internally calibrated using tryptic autolytic peaks (if present). MSMS analysis was calibrated on the fragmentation of Glu-fibrinopeptide. For MSMS analysis, the top five most intense, non-tryptic precursors were selected for fragmentation by collision-induced dissociation. Neither base-line subtraction nor smoothing was applied to recorded spectra.
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